On-Line Biomass Sensors

Solida Biotech BioMass Sensors

Risk-based stoichiometric process management, quantitative and qualitative multi-parameter process analysis which permits the gain of more process knowledge and safety as well as for TURBIDOSTAT operational mode and feedback control loop.

 

On-line measurement of total cell density

The Solida OD-CELL sensor performs online measurement of total cell density in solution. The sensor is based on optical density, which measures the turbidity of the cell suspension. The measurement is made at NIR (near-infrared) wavelengths so it is insensitive to changes in media colour.

All particles and molecules that absorb or scatter light at 880 nm will be detected, including living and dead cells and cell debris. This measurement is effective after inoculation, when cells are expanding quickly but concentrations are low, making permittivity-based readings less reliable.

Simple online measurement of cell growth
Reliable values during the growth phase
Early detection of process deviations

 

On-line measurement of viable cell density

SOLIDA LIVE-CELL sensor enables real-time, online measurement of viable cells in solution. The measurement is not influenced by changes in the media, microcarriers, dead cells, or debris. Online measurement of viable cells makes it possible to detect events and respond in real time without sampling.

  • Increase yield and lower production costs
  • Detect changes in cell physiology with frequency scanning
  • Precisely control harvesting for continuous culturing
  • Early detection of process deviations
  • Sensors optimised for multiple conductivity ranges.

The measurement principle is based on capacitance. In an alternating electrical field, viable cells behave like small capacitors. The charge from these small capacitors is measured by the sensor and reported as permittivity (capacitance per area).

Our package can include some or all of the following.

 

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Design Qualification Ensuring you know your requirements before making the purchase
Product Selection Ensuring we get you the right product at the best price
Installation and Debris Removal Ensuring the installation is complete to our standards and we recycle all packaging material
Calibration / Preventative Maintenance Ensuring the equipment is maintained and/or calibrated as per ISO17025 standards or equivalent (certificate provided after installation)
Installation Qualification Ensuring compliance is met during the installation
Operational Qualification Ensuring the equipment meets all acceptance critera when challenged against the manufacture’s specifications or the Design Qualification
Customized Performance Qualification Ensuring the equipment meets all acceptance criteria when challenged agaist your requirements
Extended Warranty with Preventative Maintenance Ensuring you are always covered no matter what and when equipment failures may occur
Scheduled Qualification Package Ensuring your equipment is continuously monitored and in compliance throughout the life cycle of the equipment
Qualification after Repair (if applicable) Ensuring the equipment stays in compliance after equipment maintenance or failures occur

OD-CELL

On-line measurement of total cell density

Sensor length 120, 225, 325, 425 mm
Diameter 25 or 12 mm
Optical path length 5 mm
Wetted parts 1.4435 stainless steel, sapphire glass, EPDM (FDA approved, USP class VI)
O-ring material EPDM (FDA approved, USP class VI)
Sterilization Steam sterilisable, maximum temperature 135° C
Pressure range 0 – 10 bar (25° C)
Measuring range 0 – 2500, equivalent to total cell density of 0.5 to 100 g/L dry weight
Measuring principal absorbance
Wavelength 880 nm (NIR)

LIVE-CELL

On-line measurement of viable cell density

Process connections DN25 a-length 70 mm (SG), 46 mm (DG), or 54 mm (DG BE) and DN12 a-length 120, 220, 320, 420 mm
Wetted parts 1.4435 stainless steel, PEEK (FDA approved, USP class VI)
O-ring material EPDM (FDA approved, USP class VI)
Sterilization Steam sterilisable, maximum temperature 135° C
Pressure range 0 – 3 bar (25° C)
Measuring range 0 – 700 pF/cm, equivalent to viable cell density of 5 to 200 g/L dry weight
Conductivity range standard 2 – 50 mS/cm; LC: 0.5 – 10 mS/cm; HC: 5 – 100 mS/cm